Code: 082-201-001
Size: 100ug / 1mg
Applications: WB,ICC/IF,ELISA,IP,Flow Cyt
Specificity: StayGold/mBaojin
Conjugate: Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
The Anti-StayGold/mBaojin Rabbit Antibody is a high-affinity recombinant antibody optimized for fluorescent protein detection, enabling sensitive and specific detection of StayGold- and mBaojin-tagged proteins.
Built on a nanobody-based VHH fused to rabbit Fc, combines the high specificity of VHH antibodies with the broad compatibility of conventional rabbit primary antibodies, making it an excellent choice for fluorescent tag antibody applications.
Validated by Western blot (WB) and ELISA, this antibody demonstrates high selectivity for StayGold and mBaojin, with no detectable cross-reactivity to unrelated proteins. Its monovalent recombinant design ensures reduced background, improved signal-to-noise ratio, and consistent performance across experiments.
It is suitable for multiple applications, including Western blot (WB), ELISA, immunocytochemistry / immunofluorescence (ICC/IF), immunoprecipitation (IP), and flow cytometry, supporting workflows such as fusion protein detection, protein expression analysis, and fluorescence imaging.
As part of our AlpHcAbs® recombinant antibody platform, this antibody provides enhanced reproducibility and batch-to-batch consistency for reliable experimental results. Explore our recombinant tag antibody collection for additional tag-specific reagents.
Immunogen: StayGold fusion protein
Clone No:A2F7
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation)
Conjugate: Unconjugated
Specificity: StayGold
Cross-Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C, (Avoid freeze / thaw cycles)
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imagingUsing antibody with Fc(mutation), the background from Fc receptors will be eliminated.
High Affinity & Specificity: Precisely detects StayGold, mStayGold, and mBaojin variants with no detectable cross-reactivity to unrelated proteins.
Monovalent Recombinant VHH-Rabbit Fc: Ensures consistent lot-to-lot performance, reduced background, and compatibility with standard secondary antibodies.
Validated Across Applications: WB, ELISA, ICC/IF, IP, Flow Cytometry.
Animal-free Production: Recombinant design eliminates variability from traditional immunization, ensuring reproducible results.
Fluorescent Protein Detection: Optimized for StayGold/mBaojin fusion proteins in imaging-based workflows.
WB: 1:5,000-1:20000
ELISA: 1:5,000-1:20000
ICC/IF: 1:100-1:1000
IP: 1-2ug/sample
Flow Cyt: 1µg for 10^6 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
FAQ
What is StayGold and mBaojin fluorescent protein?
StayGold is a next-generation green fluorescent protein known for its exceptional brightness and photostability, making it suitable for long-term imaging applications. mBaojin is a monomeric variant of StayGold, optimized for protein fusion and intracellular expression. Both are widely used as fluorescent tags for protein visualization and localization studies.
What is Anti-StayGold/mBaojin AlpHcAbs® Rabbit Antibody used for?
The Anti-StayGold/mBaojin AlpHcAbs® Rabbit Antibody is designed for the specific detection of StayGold- or mBaojin-tagged fusion proteins. It is commonly used as a primary antibody for fluorescent protein detection in applications such as Western blot (WB), ICC/IF, ELISA, IP, and Flow Cytometry.
Can this antibody be used for fluorescence imaging applications?
Yes. This antibody is suitable for fluorescence imaging-related workflows, including immunofluorescence (IF) and ICC. Its high specificity and low background make it ideal for detecting StayGold/mBaojin-tagged proteins in imaging-based experiments and protein localization studies.
Is this Anti-StayGold antibody a nanobody or a conventional antibody?
This product is a recombinant single-domain antibody (VHH) fused to rabbit IgG Fc, combining the advantages of nanobody specificity with the compatibility of rabbit primary antibodies. This design ensures consistent performance, reduced batch-to-batch variation, and compatibility with standard secondary antibodies.
Does this antibody cross-react with other fluorescent proteins?
Based on Western blot and ELISA validation, the Anti-StayGold/mBaojin AlpHcAbs® Rabbit Antibody shows high selectivity for StayGold and mBaojin, with no detectable cross-reactivity with unrelated proteins, including other commonly used fluorescent tags.
What applications has this antibody been validated for?
This antibody has been validated for: Western blot (WB) ELISA Immunocytochemistry / Immunofluorescence (ICC/IF) Immunoprecipitation (IP) Flow Cytometry It is suitable for both protein expression analysis and fluorescent protein-based detection workflows.
Is this antibody recombinant and batch consistent?
Yes. The Anti-StayGold/mBaojin AlpHcAbs® Rabbit Antibody is fully recombinant, ensuring high batch-to-batch consistency, stable supply, and reproducible experimental results, which is particularly important for long-term research projects.
What makes this antibody suitable for detecting StayGold-tagged proteins?
This antibody is engineered as a monovalent recombinant single-domain antibody (VHH) fused to rabbit IgG Fc, and its high affinity, specificity, and recombinant expression enable precise recognition of StayGold/mBaojin tags, resulting in high signal-to-noise ratios and reliable detection in both biochemical and imaging applications.
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Address:
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Western blot-Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody(082-201-001)
Lane1-6: different amount(200ng-100ng-50ng-25ng-12.5-6.25ng) of mBaojin was loaded. First antibody:Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody(082-201-001) at 0.1 µg/ml(1:10000) Secondary antibody： Anti-Rabbit IgG(Fcγ Fragment specific), AlpHcAbs® Goat antibody(HRP)(025-401-005)(1:10000)
Elisa experiment of different antigen using Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody
The plate was coated with different amounts of StayGold or its variants. 1:10000 dilution of Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody was used as the primary antibody. Anti-Rabbit IgG(H+L), AlpSdAbs® VHH(HRP) (025-102-005) was used as the secondary antibody.
Confocal experiment of StayGold-Ensconsin transfected COS-7 using Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody
StayGold-Ensconsin transfected COS-7 cells were stained with Anti-StayGold/mBaojin, AlpHcAbs® Rabbit antibody (1:500) and Goat Anti-Rabbit IgG Antibody(AF647) was the secondary antibody(1:1000). (This picture is kindly provided by Zhang Lab, School of Life Sciences, Westlake University)
Western Blot-Anti-StayGold, AlpHcAbs® Rabbit antibody
Different amount of reduced StayGold is detected by Anti-StayGold, AlpHcAbs® Rabbit antibody with 1:5000 dilution, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)(025-101-005) is used as the secondary antibody.
A titer ELISA of StayGold-Anti-StayGold, AlpHcAbs® Rabbit antibody
The plate was coated with different amounts of StayGold or its variants. 1:5000 dilution of Anti-StayGold, AlpHcAbs® Rabbit antibody was used as the detection antibody, and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(HRP)(025-101-005) is used as the secondary antibody.
