Code: 006-301-001
Size: 100ug / 1mg
Applications: ICC/IF,Flow Cyt,WB,ELISA,IP
Specificity: SUMO tag
Conjugate: Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-SUMO tag, AlpHcAbs® Mouse IgG1 antibody is designed for detecting SUMO tag fusion proteins specifically. Anti-SUMO tag, AlpHcAbs® Mouse IgG1 antibody is a monoclonal, recombinant, mouse IgG1 Fc fused single domain antibody. Based on western blot and ELISA, Anti-SUMO tag, AlpHcAbs® Mouse IgG1 antibody detects the SUMO tag selectively, no reactivity with other proteins.
Immunogen: SUMO tag
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc
Conjugate: Unconjugated
Specificity: SUMO tag
Cross-Reactivity: No cross-reactivity with other proteins
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C, (Avoid freeze / thaw cycles)
SUMO tag is short for Small Ubiquitin-like Modifier, the 100 amino acid sequence, which is necessary for regulation of protein transport and is important for controlling transcription for eukaryotic cells. Sumo tag is most frequently used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein. SUMO proteins are similar to ubiquitin in their folded structure but possess only about 20% homology to the amino acid sequence of ubiquitin. The number of SUMO genes varies among eukaryotes. SUMO proteins are conjugated to substrate proteins in a manner similar to ubiquitin. In addition, SUMO attachment can be removed by SUMO-specific isopeptidases just as UCHs and UBPs remove ubiquitin from substrate proteins.
ELISA: 1:5000 -1:20000
WB: 1:5000 -1:20000
ICC/IF: 1:200-1:1000
IP: 2ug/sample
Flow Cyt: 1:200-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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