Anti-TagFP, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 017-303-001

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,Elisa,IP

Reactivity: TagFP

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-TagFP, AlpHcAbs® Mouse IgG1 antibody is designed for detecting TagFP fusion protein specifically. Anti-TagFP, AlpHcAbs® Mouse IgG1 antibody is monovalent, recombinant single domain antibody fused to mouse IgG1 Fc. Based on immunoelectrophoresis and/or ELISA, Anti-TagFP, AlpHcAbs® Mouse IgG1 antibody is useful for detecting TagFP fusion proteins with high sensitivity.

Immunogen: TagFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: TagFP(TagRFP/TagBFP)
Cross-Reactivity: Highly selective for TagRFP/TagBFP. Does not cross-react with common GFP or dsRed derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
TagRFP is derived from the Entacmaea quadricolor fluorescent protein TurboRFP (a random mutant of eqFP578), with mutations of R162E, Q166D, S180N, F198V, F200Y at the hydrophilic interface. TagBFP was derived from TagRFP with the some mutations. TagRFP/ TagBFP has a high fluorescent quantum yield (Φfluo 0.48) and is widely used for fluorescent imaging. For biochemical analysis including mass spectrometry and enzymeactivity measurements.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:5,000-1:20,000

ELISA: 1:5,000-1:20,000

ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt: 1:200-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations