Anti-tdTomato, AlpHcAbs® Rabbit antibody

Details and Advantages

Code: 026-201-001

Specification: 100ug / 1mg

Applications: WB,ELISA,IP,ICC/IF,Flow Cyt

Reactivity: tdTomato

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-tdTomato, AlpHcAbs® Rabbit antibody is designed for detecting tdTomato fusion protein specifically. Anti-tdTomato, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-tdTomato, AlpHcAbs® Rabbit antibody reacts with the tdTomato selectively, no reactivity with other proteins.

Immunogen: tdTomato
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation)
Conjugate: Unconjugated
Specificity: tdTomato
Cross-Reactivity: Highly selective for tdTomato
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
The tandem dimer Tomato (tdTomato) is a genetically improved version of Discosoma sp. fluorescent protein (dsRed). tdTomato is an extremely bright red fluorescent protein, being one of the brightest red-shifted fluorescent proteins available and six times brighter than eGFP. One of the main advantages of fluorescent proteins is that they do not require substrates or reagents for detection, however most of them do need oxygen for the formation of their chromophores. Besides, fluorescent proteins can be measured without lysing cells, whereas this is difficult for luciferases. One of the biggest disadvantages of fluorescent reporters is that they need to overcome cellular autofluorescence. However, in general cellular autofluorescence is lower for the emission wavelength of the red channel, favoring a red fluorescent protein such as tdTomato.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:10,000-1:50,000

ELISA: 1:10,000-1:50,000

ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 10⁶ cells

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations