Anti-MBP, AlpSdAbs® VHH(HRP)

Details and Advantages

Code: 015-101-005

Specification: 100ug / 1mg

Applications: WB,ELISA

Reactivity: MBP

Conjugate: HRP

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:

Anti-MBP, AlpSdAbs® VHH(HRP) is designed for detecting MBP fusion proteins. Anti-MBP, AlpSdAbs® VHH(HRP) is based on monoclonal, recombinant, single domain antibody to MBP coupled to HRP, and Anti-MBP, AlpSdAbs® VHH(HRP) detects the MBP selectively, no reactivity with other proteins.

Immunogen: MBP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins.
Affinity: Dissociation constant KD of 0.1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.

Performance

WB: 1:5,000-1:20000

ELISA: 1:5,000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations