Anti-Myc tag, AlpSdAbs® VHH(HRP)

Details and Advantages

Code: 002-101-005

Specification: 100ug / 1mg

Applications: WB,ELISA

Reactivity: Myc tag

Conjugate: HRP

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Myc tag, AlpSdAbs® VHH(HRP) is designed for detecting Myc tag fusion proteins. Anti-Myc tag, AlpSdAbs® VHH(HRP) is based on monoclonal, recombinant, single domain antibody to Myc tag coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Myc tag, AlpSdAbs® VHH(HRP) detects the Myc tag selectively, no reactivity with other proteins.

Immunogen: Myc tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: Myc tag sequence(EQKLISEEDL)
Cross-Reactivity: Highly selective for Myc tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles), protect from light

Background:
The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance

WB: 1:5,000-1:20000

ELISA: 1:5,000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations