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Anti-StayGold/mBaojin, AlpSdAbs® VHH(HRP)

Anti-StayGold/mBaojin, AlpSdAbs® VHH(HRP)
Anti-StayGold/mBaojin, AlpSdAbs® VHH(HRP)
Anti-StayGold/mBaojin, AlpSdAbs® VHH(HRP)

Details and Advantages

Code: 082-101-005

Specification: 100ug / 1mg

Applications: WB,ELISA

Reactivity: StayGold/mBaojin

Conjugate: HRP

Advantages:

  • High lot-to-lot consistency

  • Increased sensitivity and higher affinity

  • Animal-free production

Product Detail

Product Detail

Description:
Anti-StayGold/mBaojin, AlpSdAbs® VHH(HRP) is designed for detecting StayGold/mBaojin fusion proteins. Anti-StayGold/mBaojin, AlpSdAbs® VHH(HRP) is based on monoclonal, recombinant, single domain antibody to StayGold coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-StayGold/mBaojin, AlpSdAbs® VHH(HRP) detects the StayGold/mBaojin selectively, no reactivity with other proteins.

Immunogen: StayGold fusion protein               

Clone No:A2F7      
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: StayGold
Cross-Reactivity: Recognizes StayGold, mStayGold and mBaojin variants. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C, (Avoid freeze / thaw cycles), protect from light

Background:
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.


Performance

WB:     1:5,000-1:20000
ELISA:   1:5,000-1:20000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Contact

Telephone:

+86 138 8067 6215

Address:

Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130

Western Blot-Anti-StayGold, AlpSdAbs® VHH(HRP)

Different amount of reduced StayGold is detected by Anti-StayGold, AlpSdAbs® VHH(HRP) with 1:5000 dilution.

A titer ELISA of StayGold-Anti-StayGold, AlpSdAbs® VHH(HRP)

The plate was coated with different amounts of StayGold or its variants. 1:5000 dilution of StayGold, AlpSdAbs® VHH(HRP)(082-101-005) was used as the detection antibody.