Anti-Biotin, AlpSdAbs® VHH(HRP)

Details and Advantages

Code: 078-101-005

Specification: 100ug / 1mg

Applications: WB,ELISA

Reactivity: Biotin

Conjugate: HRP

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Biotin, AlpSdAbs® VHH(HRP) is designed for detecting biotin or biotinylated protein specifically. Anti-Biotin, AlpSdAbs® VHH(HRP) is based on monovalent, recombinant single domain antibody to biotin coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Biotin, AlpSdAbs® VHH(HRP) reacts with biotin or biotinylated protein selectively, no reactivity with other antigen.

Immunogen: Biotin conjugated KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: HRP
Specificity: Biotin and biotinylated antibody, protein, peptides, oligonucleotides or solid matrices.
Cross-Reactivity: No cross-reactivity with other antigen.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light

Background:
Biotin is widely conjugated to proteins and antibodies for biochemical assays. Avidin (streptavidin)-biotin system is commonly used for many immunoassays such as ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, and Immunohistochemistry. Anti-Biotin antibody is a better alternative to avidins to minimize background and maximize signal intensity.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance

ELISA： 1:5000-1:20000
WB: 1:5000-1:20000
IHC: 1:100-1:1000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations