Code: 023-108-008
Size: 100ug / 1mg
Applications: ELISA,WB,Flow Cyt,ICC/IF
Specificity: Human IgD
Conjugate: iFluor594
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-Human IgD, AlpSdAbs® VHH(iFluor594 ×4) is designed for detecting human IgD specifically. Anti-Human IgD, AlpSdAbs® VHH(iFluor594 ×4) is based on monovalent, recombinant single domain antibody to human IgD coupled to iFluor594. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgD, AlpSdAbs® VHH(iFluor594 ×4) reacts with human IgD chain selectively, no reactivity with human IgG, IgA, IgM, IgE.
Immunogen: Human IgD
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor594(Ex: 590nm, Em: 617nm)
Specificity: Human IgD
Cross-Reactivity: Does not bind to human IgG, IgA, IgM, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles), protect from light
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
ELISA：1:5000-1:20000
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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