Code: 023-105-009
Size: 100ug / 1mg
Applications: WB,ELISA,ICC/IF,Flow Cyt
Specificity: Human IgG(CH1 Fragment specific)
Conjugate: iFluor647
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
As a leading supplier of recombinant secondary antibody, we provide this high-performance Anti-Human IgG (CH1 Fragment specific), AlpSdAbs® VHH conjugated with iFluor647 (×4). This monoclonal recombinant single-domain antibody (VHH) combines the high specificity and small size of nanobodies with the compatibility of conventional secondary antibodies, making it ideal for Western blot (WB), ELISA, immunocytochemistry/immunofluorescence (ICC/IF), Flow Cytometry, and super-resolution microscopy.
Anti-Human IgG(CH1 Fragment specific), AlpSdAbs® VHH(iFluor647 ×4) is a monoclonal recombinant vhh single domain antibody specifically designed to detect the CH1 fragment of human IgG. It is based on a monoclonal, recombinant single domain antibody to human IgG Fc coupled to iFluor647. The antibody shows high specificity for the CH1 domain of human IgG, with cross-reactivity to cynomolgus IgG but no cross-reactivity to rabbit, mouse, rat, or goat IgG.
• High Specificity: Recognizes human IgG CH1 fragment specifically; no cross-reactivity with rabbit, mouse, rat, or goat IgG; reacts with cynomolgus IgG.
• Recombinant VHH Design: Single-domain nanobody for enhanced stability, reproducibility, and reduced background.
• Fluorescent Labeling: Conjugated with iFluor647 (×4) (Ex: 651 nm, Em: 667 nm), with a defined fluorophore labeling ratio, enhancing the accuracy and reproducibility of IF experiments, for high-sensitivity fluorescence imaging.
• Animal-Free Production: Recombinant expression ensures batch-to-batch consistency and eliminates ethical concerns.
• High Affinity & Sensitivity: Delivers strong signals even at low antigen concentrations.
• Versatile Applications: Suitable for WB, ELISA, ICC/IF, Flow Cytometry, and super-resolution microscopy.
Parameter | Specification |
Immunogen | Human IgG |
Host | Alpaca |
Isotype | VHH domain of alpaca IgG2b/2c (×4) |
Conjugate | iFluor647 (×4) (Ex: 651 nm, Em: 667 nm) |
Specificity | Human IgG (CH1 Fragment specific) |
Cross-Reactivity | Reacts with cynomolgus IgG; no cross-reactivity to rabbit, mouse, rat, or goat IgG |
Purity | Recombinant expression and affinity purified |
Concentration | 1 mg/ml |
Formulation | Liquid in 10 mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% ProClin 300, 50% glycerol |
Storage | Store at –20 °C (avoid freeze/thaw cycles); protect from light |
Application | Recommended Dilution |
Western Blot (WB) | 1:5,000 – 1:20,000 |
ELISA | 1:5,000 – 1:20,000 |
ICC/IF | 1:200 – 1:2,000 |
Flow Cytometry | 1:200 – 1:2,000 |
Super-Resolution Microscopy | Optimized for CH1 fragment-containing proteins |
Notes: Optimal dilution depends on antigen density, sample type, and experimental conditions. Empirical adjustment is recommended.
Mammalian antibodies are classified into five isotypes—IgA, IgD, IgE, IgG, and IgM—determined by their respective heavy chains ($\alpha, \delta, \epsilon, \gamma$, and $\mu$). As the most prevalent isotype in human serum (70-85%), IgG comprises four subclasses (IgG1-4) and functions as a ~150 kD monomer. Each molecule consists of two heavy chains (~50 kD) and two light chains (~25 kD), structured into a functional Fc region and an antigen-binding Fab region.
VHHs are single-domain antibodies derived from Camelidae. Their ultra-small size (<15 kDa) ensures superior permeability compared to traditional fragments, offering significant advantages in diagnostic and therapeutic applications.
•Combines nanobody specificity with conventional secondary antibody compatibility
•Ideal for fluorescence imaging, WB, ELISA, ICC/IF, Flow Cytometry, and super-resolution microscopy
•Recombinant monovalent design reduces background and ensures consistent batch performance
•Optimized for researchers needing precise detection of human IgG CH1 fragments in biomedical research, diagnostics, and therapeutic development.
Looking for high-performance detection tools? Explore our full range of Recombinant Tag Antibodies and specialized Recombinant Secondary Antibodies Solutions to enhance your assay precision.
Ready to experience superior detection of human IgG CH1 fragments?
• Contact Technical Support today for pricing, samples, or technical support.
• Request COA or Quality Certificate for detailed batch information
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