Anti-Human IgG(CH1 Fragment specific), AlpSdAbs® VHH(iFluor647 ×4)
Code: 023-105-009
Specification: 100ug / 1mg
Applications: WB,ELISA,ICC/IF,Flow Cyt
Reactivity: Human IgG(CH1 Fragment specific)
Conjugate: iFluor647
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Product Detail
As a leading recombinant secondary antibodies supplier, we provide high-performance Anti-Human IgG(CH1 Fragment specific), AlpSdAbs® VHH conjugated with iFluor647 (×4) for sensitive and specific detection of human IgG CH1 fragments. This monoclonal, recombinant single-domain antibody (VHH) combines the high specificity and small molecular size of nanobodies with the compatibility of conventional secondary antibodies, making it ideal for a wide range of applications including Western blot (WB), ELISA, immunocytochemistry/immunofluorescence (ICC/IF), Flow Cytometry, and super-resolution microscopy.
Designed for researchers and laboratory professionals, this VHH-based secondary antibody ensures high lot-to-lot consistency, minimal background, and reliable signal amplification, enabling robust detection of CH1 fragment-containing human IgG molecules.
Description:
Anti-Human IgG(CH1 Fragment specific), AlpSdAbs® VHH(iFluor647 ×4) is designed for detecting human IgG CH1 fragment specifically. Anti-Human IgG(CH1 Fragment specific), AlpSdAbs® VHH(iFluor647 ×4) is based on monoclonal, recombinant single domain antibody to human IgG Fc coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Human IgG(CH1 Fragment specific), AlpSdAbs® VHH(iFluor647 ×4) reacts with the reacts with the CH1 fragment of human IgG specifically.
High specificity: Recognizes human IgG CH1 fragment with no cross-reactivity to rabbit, mouse, rat, or goat IgG; reacts with cynomolgus IgG.
Recombinant VHH design: Single-domain nanobody fused to Alpaca IgG2b/2c Fc for improved stability and reproducibility.
Fluorescent labeling: iFluor647 ×4 conjugation (Ex: 651 nm, Em: 667 nm) enables sensitive detection in fluorescence imaging applications.
Animal-free production: Eliminates ethical concerns and ensures batch-to-batch consistency.
High affinity & sensitivity: Provides strong signal even at low antigen concentrations.
Versatile applications: Suitable for WB, ELISA, ICC/IF, Flow Cytometry, and super-resolution microscopy.
Immunogen: Human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor647(Ex:651nm, Em:667nm)
Specificity: Human IgG(CH1 Fragment specific)
Cross-Reactivity: Recognizes human IgG CH1 fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles) , Protect from light.
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
| Application | Recommended Use |
|---|---|
| Western Blot (WB) | 1:5,000 – 1:20,000 |
| ELISA | 1:5,000 – 1:20,000 |
| ICC/IF | 1:200 – 1:2,000 |
| Flow Cytometry | 1:200 – 1:2,000 |
| Super-Resolution Microscopy | Optimized for imaging of CH1 fragment-containing proteins |
Notes: Optimal dilution depends on antigen density, sample type, and experimental conditions. Empirical adjustment is recommended.
Combines nanobody specificity with conventional secondary antibody compatibility
Suitable for fluorescence imaging, WB, ELISA, ICC/IF, Flow Cytometry, and super-resolution microscopy
Recombinant and monovalent design ensures reduced background, consistent batch performance, and reliable detection
Optimized for researchers requiring precise detection of human IgG CH1 fragments in biomedical research, diagnostics, and therapeutic studies
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