Code: 023-113-005
Size: 100ug / 1mg
Applications: WB,ELISA
Specificity: Human IgG
Conjugate: HRP
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-Human IgG(CH3 Fragment specific), AlpSdAbs® VHH(HRP) a human IgG CH3 fragment detection antibody designed for selective recognition of human IgG CH3 fragment. This monovalent, recombinant single-domain VHH antibody derived from alpaca heavy chain variable regions is directly conjugated to HRP. Based on immunoelectrophoresis and/or ELISA, it reacts exclusively with human IgG CH3 fragment and shows no reactivity with rabbit, mouse, rat, or goat IgG.
Immunogen | Human IgG |
Host | Alpaca |
Isotype | VHH domain of alpaca IgG2b/2c |
Conjugate | HRP |
Specificity | Human IgG CH3 fragment |
Cross-Reactivity | Cross-react with cynomolgus IgG, No cross-reactivity with rabbit, mouse, rat, goat IgG |
Purity | Recombinant Expression and Affinity purified |
Concentration | 1 mg/ml |
Formulation | Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol |
Storage | Store at –20 °C (Avoid freeze / thaw cycles) |
✅ Highly specific detection: Reacts exclusively with human IgG CH3 fragment, ensuring no cross-reaction with common lab species IgGs. Learn more in our Recombinant Antibody page.
✅ Small, permeable VHH format: Recombinant single-domain antibody (<15 kDa) derived from alpaca heavy chain variable regions.
✅ Convenient HRP conjugation: Directly coupled for rapid immunoassays, suitable for ELISA and other detection platforms.
✅ High purity and batch consistency: Recombinant expression and affinity purification guarantee reproducible results.
✅ Broad applicability: Cross-reacts with cynomolgus IgG for related non-human primate studies.
ELISA: 1:5000-1:20000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Antibodies in mammals are classified into five main isotypes: IgA, IgD, IgE, IgG, and IgM, based on heavy chain type: alpha, delta, epsilon, gamma, or mu. IgG is the most abundant antibody in human serum, accounting for 70–85% of total immunoglobulins, and includes four subclasses (IgG1–IgG4).
The IgG molecule consists of Fab and Fc regions, two heavy chains (~50 kDa each) and two light chains (~25 kDa each). Heavy chains include a variable domain (VH) and three constant domains (CH1, CH2, CH3). Monomeric IgG has a molecular weight of ~150 kDa.
VHH antibodies are single-domain antibodies derived from camelid heavy-chain variable regions. With a size <15 kDa, VHH antibodies exhibit superior permeability and stability, making them highly valuable for research, diagnostics, and therapeutic development.
· Order Now to start your specific detection of human IgG CH3 fragment
· Request COA or Quality Certificate for detailed batch information
· Contact Technical Support for dilution recommendations or application guidance
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A titer ELISA of different IgG or domain-Anti-Human IgG(CH3 Fragment specific), AlpSdAbs® VHH(HRP)
The plate was coated with different amounts of IgG or domain(ng/ml). 1:5000 dilution of Anti-Human IgG(CH3 Fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody.
A titer ELISA of different IgG or domain-Anti-Human IgG(CH3 Fragment specific), AlpSdAbs® VHH(HRP)
The plate was coated with different amounts of IgG or domain(ng/ml). 1:5000 dilution of Anti-Human IgG(CH3 Fragment specific), AlpSdAbs® VHH(HRP) was used as the detection antibody.
