Code: 001-107-009
Size: 100ug / 1mg
Applications: ICC/IF,ELISA,Flow Cy,WB
Specificity: Mouse IgM
Conjugate: iFluor647
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(iFluor647 ×4) is designed for detecting mouse IgM specifically. Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(iFluor647 ×4) is based on monoclonal, recombinant single domain antibody to mouse IgM(µ chain specific) coupled to iFluor647. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(iFluor647 ×4) reacts with the µ chain of mouse IgM selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG.
Immunogen: Recombinant Fc region of mouse IgM
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFlour647(Ex: 652nm, Em: 668nm)
Specificity: Fc region of mouse IgM(µ chain specific)
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles), protect from light
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
ELISA：1:5000-1:20000
WB: 1:5000-1:20000
Flow Cyt: 1:100-1:1000
ICC/IF： 1:100-1:1000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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