Code: 023-408-001
Size: 100ug / 1mg
Applications: ELISA,IP,ICC/IF,Flow Cyt
Specificity: Human IgD
Conjugate: Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Description:
Anti-Human IgD, AlpHcAbs® Goat antibody is designed for detecting human IgD specifically. Anti-Human IgD, AlpHcAbs® Goat antibody is monovalent, recombinant single domain antibody fused to goat IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Human IgD, AlpHcAbs® Goat antibody reacts with human IgD selectively, no reactivity with human IgG, IgA, IgM, IgE.
Immunogen: Human IgD
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: Unconjugated
Specificity: Human IgD
Cross-Reactivity: Does not bind to human IgG, IgA, IgM, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Application:
ELISA: 1:10000-1:50000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt: 1µg for 106 cells
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empiricall
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