Anti-Rabbit IgG(Fab Fragment specific), AlpHcAbs® Goat antibody(Biotin)

Details and Advantages

Code: 025-403-004

Specification:

Applications: WB,ELISA,IP,Flow Cyt

Reactivity: Rabbit IgG(Fab Fragment specific)

Conjugate: Biotin

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Rabbit IgG(Fab Fragment specific), AlpHcAbs® Goat antibody(Biotin) is designed for detecting Fc region of rabbit IgG specifically. Anti-Rabbit IgG(Fab Fragment specific), AlpHcAbs® Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to Fab region of rabbit IgG coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG(Fab Fragment specific), AlpHcAbs® Goat antibody(Biotin) reacts with the Fab fragment of rabbit IgG selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.

Immunogen: Recombinant Rabbit IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: Biotin-SP (long spacer)
Specificity: Fab region of Rabbit IgG
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.