Code: 023-101-004
Size: 100ug / 1mg
Applications: WB,ELISA,Purification,BLI,SPR
Specificity: Human IgG(Fcγ Fragment specific)
Conjugate: Biotin
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Description:
Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(Biotin) is designed for detecting human IgG Fcγ fragment specifically. Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant single domain antibody to human IgG Fc coupled to Biotin, and Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(Biotin) reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG.
Immunogen: Recombinant Fc region of human IgG
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Human IgG(Fcγ fragment specific)
Cross-Reactivity: Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Performance
ELISA: 1:10,000-1:50,000
WB: 1:10,000-1:50,000
IP: 1-2ug/sample
BLI (biolayer interferometry)
SPR (surface plasmon resonance)
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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Contact
Telephone:
+86 17882311198
Address:
Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130
Email:
ELISA of specificity for different species of IgG-Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(Biotin)
ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin(067-101-005) as the secondary antibody.
A titer ELISA of human IgG-Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH (Biotin)
A titer ELISA of human IgG. The plate was coated with different amounts of human IgG. 1:10000 dilution of Anti-Human IgG(Fcγ fragment specific), AlpSdAbs® VHH (Biotin) was used as the primary antibody. An HRP conjugated streptavidin(067-101-005) as the secondary antibody.
