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Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin)

Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin)
Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin)
Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin)

Details and Advantages

Code: 001-107-004

Specification: 100ug / 1mg

Applications: ELISA,Purification,BLI,SPR

Reactivity: Mouse IgM(µ chain specific)

Conjugate: Biotin

Advantages:

  • High lot-to-lot consistency

  • Increased sensitivity and higher affinity

  • Animal-free production

Product Detail

Product Detail

Description: 

Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH (Biotin) is designed for detecting mouse IgM specifically. Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH (Biotin) is based on monoclonal, recombinant single domain antibody to mouse IgM(µ chain specific) coupled to Biotin, and Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH (Biotin) reacts with the µ chain of mouse IgM selectively, no reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG.


Immunogen: Recombinant Fc region of mouse IgM                
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c  
Conjugate: Biotin-SP (long spacer)  
Specificity: Fc region of mouse IgM(µ chain specific)
Cross-Reactivity: No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG       
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at -20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C


Performance

ELISA:    1:5000-1:20000
WB:       1:5000-1:20000

IP:         1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Contact

Telephone:

+86 138 8067 6215

Address:

Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130

A titer ELISA of mouse IgM-Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin)

A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. 1:5000 dilution of Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin(067-101-005) as the secondary antibody.

ELISA of specificity for different species of IgG-Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin)

ELISA of specificity for different species of IgG. The plate was coated with 2ug/ml of different IgG. 1:1000 dilution of Anti-Mouse IgM(µ chain specific), AlpSdAbs® VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin(067-101-005) as the secondary antibody.