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Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)

Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)
Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)
Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)

Details and Advantages

Code: 025-101-004

Specification: 100ug / 1mg

Applications: WB,ELISA,Purification,BLI,SPR

Reactivity: Rabbit IgG(Fcγ Fragment specific)

Conjugate: Biotin

Advantages:

  • High lot-to-lot consistency

  • Increased sensitivity and higher affinity

  • Animal-free production

Product Detail

Product Detail

Description: 

Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) is designed for detecting Fc region of rabbit IgG specifically. Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) is based on monovalent, recombinant single domain antibodies to rabbit IgG (Fcγ Fragment specific) coupled to biotin, and the Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) reacts with the Fc region of rabbit IgG selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.


Immunogen: Recombinant Rabbit IgG                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c    
Conjugate: Biotin-SP (long spacer)
Specificity: Fc region of Rabbit IgG
Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG
Affinity: <1nM      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at -20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Performance

ELISA:  1:10000-1:50000
WB:     1:10000-1:50000

IP:        1-2ug/sample

BLI (biolayer interferometry)

SPR (surface plasmon resonance)


Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

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Contact

Telephone:

+86 138 8067 6215

Address:

Building 16, No.99 Haiwang Road, Wenjiang, Chengdu, China 611130

ELISA of specificity for different species of IgG-Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)

ELISA of specificity for different species of IgG. Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin(067-101-005) as the secondary antibody.

A titer ELISA of Rabbit IgG-Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin)

A titer ELISA of Rabbit IgG. The plate was coated with different amounts of Rabbit IgG. 1:10000 dilution of Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(Biotin) was used as the primary antibody. An HRP conjugated streptavidin(067-101-005) as the secondary antibody.