Code: 097-201-001
Size: 100ug / 1mg
Applications: WB,ICC/IF,IHC,Flow Cyt
Specificity: GGGGS Linker
Conjugate: Unconjugated
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Product Detail
Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody is designed for detecting GGGGS linker specifically. Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody is recombinant antibody. Based on immunoelectrophoresis and/or ELISA, Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody reacts with GGGGS linker and their fusion proteins.
Immunogen: Synthetic peptide(GGGGSGGGGSGGGGS)
Host: Rabbit
Isotype: Rabbit IgG
Conjugate: Unconjugated
Specificity: This product recognizes (GnS)m linker(n≥2, m≥2) in proteins, antibodies or cells, such as (G2S)2 linker, (G2S)4 linker, (G3S)3 linker, (G4S)2 linker and (G4S)3 linker, etc.
Cross-Reactivity: N/A
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at -20 °C(Avoid freeze / thaw cycles)
As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins. GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid. Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv(ScFv) domains expressed on the surfaces of CAR-T cells.
Flow Cyt: 1:100-1:400
WB: 1:2000-1:4000
IHC: 1:4000-1:10000
IF/ICC: 1:1000-1:2000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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Flow cytometry experiment of Transfected HEK-293T using Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody
1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody(1:100) and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8) ( Cat No.025-101-009) (red), or Rabbit IgG Isotype Control(blue).
Flow cytometry experiment of Transfected HEK-293T using Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody
1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with Anti-GGGGS Linker, AlpHcAbs® Rabbit antibody(1:100) and Anti-Rabbit IgG(Fcγ Fragment specific), AlpSdAbs® VHH(iFluor647 ×8) ( Cat No.025-101-009) (red), or Rabbit IgG Isotype Control(blue).
