Anti-Human IgM, AlpHcAbs® Goat antibody

Details and Advantages

Code: 023-410-001

Specification:

Applications: ICC/IF,ELISA,IP,Flow Cyt

Reactivity: Human IgM

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Human IgM, AlpHcAbs® Goat antibody is designed for detecting human IgM specifically. Anti-Human IgM, AlpHcAbs® Goat antibody is monovalent, recombinant single domain antibody fused to goat IgG Fc(mutation). Based on immunoelectrophoresis and/or ELISA, Anti-Human IgM, AlpHcAbs® Goat antibody reacts with human IgM selectively, no reactivity with human IgG, IgD, IgA, IgE.

Immunogen: Human IgM
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Conjugate: Unconjugated
Specificity: Human IgM
Cross-Reactivity: Does not bind to human IgG, IgD, IgA, IgE
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
In mammals, antibodies are classified into five main classes or isotypes–IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.