Anti-mScarlet3-H/mYongHong, AlpHcAbs® Rabbit antibody

Details and Advantages

Code: 028-201-001

Specification: 100ug / 1mg

Applications: WB,ELISA,IP,ICC/IF,Flow Cyt

Reactivity: mScarlet3-H/mYongHong

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-mScarlet3-H, AlpHcAbs® Rabbit antibody is designed for detecting mScarlet fusion protein specifically. Anti-mScarlet3-H, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-mScarlet3-H, AlpHcAbs® Rabbit antibody reacts with the mScarlet selectively, no reactivity with other proteins.

Immunogen: mScarlet
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit Fc(mutation)
Conjugate: Unconjugated
Specificity: mScarlet,mScarlet3,mScarlet3-H
Cross-Reactivity: Highly selective for mScarlet
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
The fluorescent protein mScarlet is a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:10,000-1:50,000
ELISA: 1:10,000-1:50,000
ICC/IF: 1:200-1:1000
IP: 1-2ug/sample
Flow Cyt： 1µg for 10^6 cells

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations