Anti-SUMO tag, AlpHcAbs® Rabbit antibody(iFluor647)

Details and Advantages

Code: 006-201-009

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,ELISA,IP

Reactivity: SUMO tag

Conjugate: iFluor647

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-SUMO tag, AlpHcAbs® Rabbit antibody(iFluor647) is designed for detecting SUMO tag fusion proteins specifically. Anti-SUMO tag, AlpHcAbs® Rabbit antibody(iFluor647) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to SUMO tag coupled to iFluor647. Based on immunofluorescence and/or ELISA, Anti-SUMO tag, AlpHcAbs® Rabbit antibody(iFluor647) detects the SUMO tag selectively, no reactivity with other proteins.

Immunogen: SUMO tag
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation)
Conjugate: iFluor647(Ex: 651nm, Em:667nm), 3 moles iFluor647 per mole IgG
Specificity: SUMO tag
Cross-Reactivity: No cross-reactivity with other proteins
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C (Avoid freeze / thaw cycles), protect from light

Background:
SUMO tag is short for Small Ubiquitin-like Modifier, the 100 amino acid sequence, which is necessary for regulation of protein transport and is important for controlling transcription for eukaryotic cells. Sumo tag is most frequently used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein. SUMO proteins are similar to ubiquitin in their folded structure but possess only about 20% homology to the amino acid sequence of ubiquitin. The number of SUMO genes varies among eukaryotes. SUMO proteins are conjugated to substrate proteins in a manner similar to ubiquitin. In addition, SUMO attachment can be removed by SUMO-specific isopeptidases just as UCHs and UBPs remove ubiquitin from substrate proteins.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:5000 -1:20000
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations