Anti-mGold, AlpHcAbs® Mouse IgG2a antibody(iFluor647)

Details and Advantages

Code: 061-301-009

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,ELISA,IP

Reactivity: mGold

Conjugate: iFluor647

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-mGold, AlpHcAbs® Mouse IgG2a antibody(iFluor647) is designed for detecting mGold fusion proteins specifically. Anti-mGold, AlpHcAbs® Mouse IgG2a antibody(iFluor647) is based on monoclonal, recombinant, mouse IgG2a Fc fused single domain antibody to mGold coupled to iFluor647. Based on immunofluorescence and/or ELISA, Anti-mGold, AlpHcAbs® Mouse IgG2a antibody(iFluor647) detects the mGold selectively, no reactivity with other proteins.

Immunogen: mGold
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG2a Fc(mutation)
Conjugate: iFluor647(Ex: 651nm, Em:667nm), 3 moles iFluor647 per mole IgG
Specificity: mGold, mGold2s, mGold2t
Cross-Reactivity: Recognizes GFP and YFP variants
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C (Avoid freeze / thaw cycles), protect from light

Background:
mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

Performance

WB: 1:5000 -1:20000
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations