Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2a antibody(APC)

Details and Advantages

Code: 016-313-011

Specification:

Applications: ICC/IF,Flow Cyt

Reactivity: DYKDDDDK tag

Conjugate: APC

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2a antibody(APC) is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2a antibody(APC) is based on recombinant, mouse IgG2a Fc fused fab of alpaca IgG1 to DYKDDDDK tag fused to APC. Based on western blot and ELISA, Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2a antibody(APC) reacts with the DYKDDDDK tag selectively, no reactivity with other proteins.

Immunogen: DYKDDDDK tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to mouse IgG2a Fc(mutation)
Conjugate: APC(Ex: 651nm, Em: 662nm)
Specificity: DYKDDDDK tag sequence
Cross-Reactivity: Highly selective for DYKDDDDK tag sequence
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at 2-8 °C, Protect from light.

Background:
The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.