Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 016-323-001

Specification:

Applications: WB,ICC/IF,ELISA,IP,Flow Cyt

Reactivity: DYKDDDDK tag

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG1 antibody is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG1 antibody is recombinant fab of alpaca IgG1 fused to mouse IgG1 Fc. Based on western blot and ELISA, Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG1 antibody reacts with the DYKDDDDK tag selectively, no reactivity with other proteins.

Immunogen: DYKDDDDK tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: DYKDDDDK tag sequence
Cross-Reactivity: Highly selective for DYKDDDDK tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.