Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(iFluor594)

Details and Advantages

Code: 016-303-008

Specification:

Applications: WB,ICC/IF,ELISA,Flow Cyt

Reactivity: DYKDDDDK tag

Conjugate: iFluor594

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(iFluor594) is designed for detecting DYKDDDDK tag fusion protein specifically. Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(iFluor594) is based on recombinant, IgG2b Fc fused fab of alpaca IgG1 to DYKDDDDK tag coupled to iFluor594. Based on western blot and ELISA, Anti-DYKDDDDK tag, AlpHcAbs® Mouse IgG2b antibody(iFluor594) reacts with the DYKDDDDK tag selectively, no reactivity with other proteins.

Immunogen: DYKDDDDK tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation)
Conjugate: iFluor594(Ex: 592nm, Em: 614nm), 3 moles iFluor594 per mole IgG
Specificity: DYKDDDDK tag sequence
Cross-Reactivity: Highly selective for DYKDDDDK tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C (Avoid freeze / thaw cycles), protect from light

Background:
The DYKDDDDK peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, DYKDDDDK tag is unlikely to affect the tagged protein’s biochemical properties. DYKDDDDK tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.