Anti-HA tag, AlpHcAbs® Rabbit IgG antibody(APC)

Details and Advantages

Code: 003-201-011

Specification:

Applications: ICC/IF,Flow Cyt

Reactivity: HA tag

Conjugate: APC

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-HA tag, AlpHcAbs® Rabbit IgG antibody(APC) is designed for detecting HA tag fusion proteins specifically. Anti-HA tag, AlpHcAbs® Rabbit IgG antibody(APC) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to HA tag fused to APC. Based on immunofluorescence and/or ELISA, Anti-HA tag, AlpHcAbs® Rabbit IgG antibody(APC) detects the HA tag selectively, no reactivity with other proteins.

Immunogen: HA tag fused KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to rabbit IgG Fc(mutation)
Conjugate: APC(Ex: 651nm, Em: 662nm)
Specificity: HA tag(YPYDVPDYA)
Cross-Reactivity: Highly selective for HA tag sequence
Purity: Recombinant Expression and Affinity purified
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at 2-8 °C, Protect from light.

Background:
The HA tag is widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, HA tag is unlikely to affect the tagged protein’s biochemical properties. HA tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.