Anti-Halo Tag, AlpHcAbs® Rabbit antibody

Details and Advantages

Code: 012-201-001

Specification:

Applications: WB,ELISA,IP

Reactivity: Halo tag

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:

Anti-Halo Tag, AlpHcAbs® Rabbit antibody is designed for detecting Halo tag fusion proteins. Anti-Halo Tag, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-Halo Tag, AlpHcAbs® Rabbit antibody detects the Halo tag selectively, no reactivity with other proteins.

Immunogen: Halo-KLH
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc
Conjugate: Unconjugated
Specificity: Halo tag
Cross-Reactivity: No cross-reactivity with other proteins
Affinity: Dissociation constant KD of 1nM
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: PBS pH=7.510mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: store at –20 °C. Avoid freeze / thaw cycle

Background:

The protein tag(Halo tag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (Halo tag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces. Due to the single-chain nature of sdAbs and their increased sensitivity and higher affinity to Halo tag fusion proteins, Anti-Halo Tag, AlpHcAbs® Rabbit antibody can be used to detect the Halo tag selectively, with high sensitivity and negligible non-specific background.

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