Anti-His tag, AlpHcAbs® Mouse IgG2a antibody(PE)

Details and Advantages

Code: 004-312-010

Specification:

Applications: ICC/IF,Flow Cyt

Reactivity: His tag

Conjugate: PE

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-His tag, AlpHcAbs® Mouse IgG2a antibody(PE) is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpHcAbs® Mouse IgG2a antibody(PE) is based on monoclonal, recombinant, mouse IgG2a Fc fused fab of alpaca IgG1 to His tag fused to PE, and Anti-His tag, AlpHcAbs® Mouse IgG2a antibody(PE) detects the His tag selectively, no reactivity with other proteins.

Immunogen: 6*His tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG2a Fc(mutation)
Conjugate: PE(Ex: 488nm, Em: 575nm)
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Purity: Recombinant expression and Affinity purified
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at 2-8 °C, Protect from light.

Background:
The HHHHHH peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein’s biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.