Anti-His tag, AlpHcAbs® Mouse IgG2b antibody

Details and Advantages

Code: 004-322-001

Specification:

Applications: WB,ICC/IF,ELISA,IP,Flow Cyt

Reactivity: His tag,His tag

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-His tag, AlpHcAbs® Mouse IgG2b antibody is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpHcAbs® Mouse IgG2b antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2b Fc. Based on western blot and ELISA, Anti-His tag, AlpHcAbs® Mouse IgG2b antibody detects the His tag selectively, no reactivity with other proteins.

Immunogen: 6*His tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation)
Conjugate: Unconjugated
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
The HHHHHH peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein’s biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.