Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor647)

Details and Advantages

Code: 004-202-009

Specification:

Applications: WB,ICC/IF,ELISA,Flow Cyt

Reactivity: His tag,His tag

Conjugate: iFluor647

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor647) is designed for detecting His tag fusion proteins specifically. Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor647) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 to His tag coupled to iFluor647, and Anti-His tag, AlpHcAbs® Rabbit antibody(iFluor647) detects the His tag selectively, no reactivity with other proteins.

Immunogen: 6*His tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Rabbit Fc(mutation)
Conjugate: iFluor647(Ex: 651nm, Em:667nm), 3 moles iFluor647 per mole IgG
Specificity: His tag sequence(HHHHHH)
Cross-Reactivity: Highly selective for His tag sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C (Avoid freeze / thaw cycles), protect from light

Background:
The HHHHHH peptide are widely used for detecting, manipulating or purifying proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, His tag is unlikely to affect the tagged protein’s biochemical properties. His tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.