Anti-MBP, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 015-301-001

Specification:

Applications: ICC/IF,Flow Cyt,WB,Elisa,IP

Reactivity: MBP

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-MBP, AlpHcAbs® Mouse IgG1 antibody is designed for detecting MBP fusion protein specifically. Anti-MBP, AlpHcAbs® Mouse IgG1 antibody is monovalent, recombinant single domain antibody fused to mouse IgG1 Fc. Based on immunoelectrophoresis and/or ELISA, Anti-MBP, AlpHcAbs® Mouse IgG1 antibody is useful for detecting MBP fusion proteins with high sensitivity.

Immunogen: MBP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

FAQ

Citations

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