Anti-MBP, AlpHcAbs® Rabbit antibody

Anti-MBP, AlpHcAbs® Rabbit antibody
Anti-MBP, AlpHcAbs® Rabbit antibody

Details and Advantages

Code: 015-201-001

Specification:

Applications: WB,ELISA,IP

Reactivity: MBP

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Detail

Product Detail

Description: 

Anti-MBP, AlpHcAbs® Rabbit antibody is designed for detecting MBP fusion protein specifically. Anti-MBP, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc, and the Anti-MBP, AlpHcAbs® Rabbit antibody is useful for detecting MBP fusion proteins with high sensitivity.

Immunogen: MBP fusion protein                  
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc  
Conjugate:  Unconjugated
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins.
Affinity: Dissociation constant KD of 0.1nM      
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.



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