Anti-Myc tag, AlpHcAbs® Mouse IgG2b antibody

Details and Advantages

Code: 002-323-001

Specification:

Applications: WB,ICC/IF,ELISA,IP,Flow Cyt

Reactivity: Myc tag

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Myc tag, AlpHcAbs® Mouse IgG2b antibody is designed for detecting Myc tag fusion protein specifically. Anti-Myc tag, AlpHcAbs® Mouse IgG2b antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2b Fc. Based on western blot and ELISA, Anti-Myc tag, AlpHcAbs® Mouse IgG2b antibody reacts with the Myc tag sequence(EQKLISEEDL) selectively, no reactivity with other proteins.

Immunogen: Myc tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Mouse IgG2b Fc(mutation)
Conjugate: Unconjugated
Specificity: Myc tag sequence(EQKLISEEDL)
Cross-Reactivity: Highly selective for Myc tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.