Anti-Myc tag, AlpHcAbs® Rabbit antibody(iFluor647)

Details and Advantages

Code: 002-203-009

Specification:

Applications: WB,ICC/IF,ELISA,Flow Cyt

Reactivity: Myc tag

Conjugate: iFluor647

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Myc tag, AlpHcAbs® Rabbit antibody(iFluor647) is designed for detecting Myc tag fusion proteins specifically. Anti-Myc tag, AlpHcAbs® Rabbit antibody(iFluor647) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 antibody to Myc tag coupled to iFluor647, and Anti-Myc tag, AlpHcAbs® Rabbit antibody(iFluor647) detects the Myc tag selectively, no reactivity with other proteins.

Immunogen: Myc tag fusion protein
Host: Alpaca pacous
Isotype: Fab of alpaca IgG1 fused to Rabbit Fc(mutation)
Conjugate: iFluor647(Ex: 651nm, Em:667nm), 3 moles iFluor647 per mole IgG
Specificity: Myc tag sequence(EQKLISEEDL)
Cross-Reactivity: Highly selective for Myc tag sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), protect from light

Background:
The Myc peptide are widely used for detecting or manipulating proteins. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, Myc tag is unlikely to affect the tagged protein’s biochemical properties. Myc tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.