Anti-Strep-tag II, AlpHcAbs® Rabbit antibody(Biotin)

Details and Advantages

Code: 062-203-004

Specification:

Applications: WB,ELISA,IP

Reactivity: Strep-tag II

Conjugate: Biotin

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Strep-tag II, AlpHcAbs® Rabbit antibody(Biotin) is designed for detecting Strep-tag II fusion proteins specifically. Anti-Strep-tag II, AlpHcAbs® Rabbit antibody(Biotin) is based on monoclonal, recombinant, rabbit Fc fused Fab of alpaca IgG1 antibody to Strep-tag II coupled to Biotin, and Anti-Strep-tag II, AlpHcAbs® Rabbit antibody(Biotin) detects the Strep-tag II selectively, no reactivity with other proteins.

Immunogen: Strep-tag II fusion protein
Host: Alpaca pacous
Isotype: Fab region of alpaca IgG1 fused to Rabbit Fc(mutation)
Conjugate: Biotin
Specificity: Strep-tag II sequence(WSHPQFEK)
Cross-Reactivity: Highly selective for Strep-tag II sequence
Purity: Recombinant expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C, (Avoid freeze / thaw cycles)

Background:
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag(AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II(WSHPQFEK).
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.