Anti-V5 tag, AlpHcAbs® Mouse IgG2b antibody

Details and Advantages

Code: 064-321-001

Specification:

Applications: WB,ICC/IF,ELISA,IP,Flow Cyt

Reactivity: V5 tag

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-V5 tag, AlpHcAbs® Mouse IgG2b antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Mouse IgG2b antibody is monovalent, recombinant single domain antibody fused to mouse IgG2b Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Mouse IgG2b antibody reacts with the V5 tag sequence(GKPIPNPLLGLDST) selectively, no reactivity with other proteins.

Immunogen: V5 tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Mouse IgG2b Fc(mutation)
Conjugate: Unconjugated
Specificity: V5 tag sequence(GKPIPNPLLGLDST)
Cross-Reactivity: Highly selective for V5 tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Background:
The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.