Anti-V5 tag, AlpHcAbs® Rabbit antibody(HRP)

Details and Advantages

Code: 064-201-005

Specification:

Applications: WB,ELISA

Reactivity: V5 tag

Conjugate: HRP

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-V5 tag, AlpHcAbs® Rabbit antibody(HRP) is designed for detecting V5 tag fusion proteins specifically. Anti-V5 tag, AlpHcAbs® Rabbit antibody(HRP) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to V5 tag coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-V5 tag, AlpHcAbs® Rabbit antibody(HRP) detects the V5 tag selectively, no reactivity with other proteins.

Immunogen: V5 tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc(mutation)
Conjugate: HRP
Specificity: V5 tag sequence(GKPIPNPLLGLDST)
Cross-Reactivity: Highly selective for V5 tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light

Background:
The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.