Anti-turboGFP, AlpHcAbs® Mouse IgG1 antibody

Details and Advantages

Code: 014-301-001

Specification:

Applications: ICC/IF,Flow Cyt,Elisa,IP

Reactivity: turboGFP

Conjugate: Unconjugated

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-turboGFP, AlpHcAbs® Mouse IgG1 antibody is designed for detecting turboGFP fusion protein specifically. Anti-turboGFP, AlpHcAbs® Mouse IgG1 antibody is monovalent, recombinant single domain antibody fused to mouse IgG1 Fc. Based on immunoelectrophoresis and/or ELISA, Anti-turboGFP, AlpHcAbs® Mouse IgG1 antibody is useful for detecting turboGFP fusion proteins with high sensitivity.

Immunogen: TurboGFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to mouse IgG1 Fc(mutation)
Conjugate: Unconjugated
Specificity: TurboGFP
Cross-Reactivity: No cross-reactivity with CopGFP, jellyfish GFP and derivatives
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata. It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20% sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies do not bind to TurboGFP. TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.

FAQ

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