Anti-MBP, AlpSdAbs® VHH(Biotin)

Details and Advantages

Code: 015-101-004

Specification:

Applications: ELISA,Purification,BLI,SPR

Reactivity: MBP

Conjugate: Biotin

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:

Anti-MBP, AlpSdAbs® VHH(Biotin) is designed for detecting MBP fusion proteins. Anti-MBP, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to MBP coupled to Biotin, and Anti-MBP, AlpSdAbs® VHH(Biotin) detects the MBP selectively, no reactivity with other proteins.

Immunogen: MBP fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin
Specificity: MBP
Cross-Reactivity: Recognizes MBP specifically. Does not cross-react with other proteins.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:

MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. The mechanism by which MBP increases solubility is not well understood. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins.

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