Anti-Rat IgG(Fab Fragment specific), AlpSdAbs® VHH(Biotin)

Details and Advantages

Code: 071-103-004

Specification:

Applications: WB,ELISA,IP,Purification,BLI,SPR

Reactivity: Rat IgG(Fab Fragment specific)

Conjugate: Biotin

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-Rat IgG(Fab Fragment specific), AlpSdAbs® VHH(Biotin) is designed for detecting rat IgG fab fragment specifically. Anti-Rat IgG(Fab Fragment specific), AlpSdAbs® VHH(Biotin) is based on recombinant single domain antibody to rat IgG fab fragment coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Rat IgG(Fab Fragment specific), AlpSdAbs® VHH(Biotin) reacts with rat IgG fab fragment selectively, no reactivity with mouse IgG, rabbit IgG, goat IgG, human IgG.

Immunogen: Rat IgG Fab
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: Biotin-SP (long spacer)
Specificity: Rat IgG(Fcγ Fragment specific)
Cross-Reactivity: Does not bind to mouse IgG, rabbit IgG, goat IgG, human IgG
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage: Store at –20 °C(Avoid freeze / thaw cycles)

Background:
There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from rat. Each isotype has a different heavy chain. Rat IgG consists of four subclasses-IgG1, IgG2a, IgG2b, IgG2c. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

FAQ

Citations