Anti-V5 tag, AlpSdAbs® VHH(iFluor488)

Details and Advantages

Code: 064-101-007

Specification:

Applications: WB,ICC/IF,ELISA,Flow Cyt

Reactivity: V5 tag

Conjugate: iFluor488

Advantages:

High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-V5 tag, AlpSdAbs® VHH(iFluor488) is designed for detecting V5 tag fusion proteins. Anti-V5 tag, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to V5 tag coupled to iFluor488. Based on immunoelectrophoresis and/or ELISA, Anti-V5 tag, AlpSdAbs® VHH(iFluor488) detects the V5 tag selectively, no reactivity with other proteins.

Immunogen: V5 tag fusion protein
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor488(Ex: 495nm, Em: 519nm)
Specificity: V5 tag sequence(GKPIPNPLLGLDST)
Cross-Reactivity: Highly selective for V5 tag sequence
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300,50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light

Background:
The V5 tag is a 14 amino acid peptide derived from a small epitope on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. Because of its small size, V5 tag is unlikely to affect the tagged protein’s biochemical properties. V5 tag is useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

FAQ

Citations