Anti-SUMO tag, AlpSdAbs® VHH(iFluor594)

Details and Advantages

Code: 006-101-008

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,WB,ELISA

Reactivity: SUMO tag

Conjugate: iFluor594

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-SUMO tag, AlpSdAbs® VHH(iFluor594) is designed for detecting SUMO tag fusion proteins specifically. Anti-SUMO tag, AlpSdAbs® VHH(iFluor594) is based on monoclonal, recombinant, single domain antibody to SUMO tag coupled to iFluor594. Based on immunofluorescence and/or ELISA, Anti-SUMO tag, AlpSdAbs® VHH(iFluor594) detects the SUMO tag selectively, no reactivity with other proteins.

Immunogen: SUMO tag fused GFP
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor594(Ex: 592nm, Em: 614nm)
Specificity: SUMO tag
Cross-Reactivity: No cross-reactivity with other proteins
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, protect from light

Background:
SUMO tag is short for Small Ubiquitin-like Modifier, the 100 amino acid sequence, which is necessary for regulation of protein transport and is important for controlling transcription for eukaryotic cells. Sumo tag is most frequently used as N-end fusion sequence in yeast to increase the expression and solubility of the desired recombinant protein. SUMO proteins are similar to ubiquitin in their folded structure but possess only about 20% homology to the amino acid sequence of ubiquitin. The number of SUMO genes varies among eukaryotes. SUMO proteins are conjugated to substrate proteins in a manner similar to ubiquitin. In addition, SUMO attachment can be removed by SUMO-specific isopeptidases just as UCHs and UBPs remove ubiquitin from substrate proteins.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance

WB: 1:5,000-1:20000
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Super-resolution microscopy

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations