Anti-mGold, AlpSdAbs® VHH(iFluor488)

Details and Advantages

Code: 061-101-007

Specification: 100ug / 1mg

Applications: ICC/IF,Flow Cyt,ELISA

Reactivity: mGold

Conjugate: iFluor488

Advantages:

High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production

Product Description Manual MSDS FAQ Citations

Product Detail

Description:
Anti-mGold, AlpSdAbs® VHH(iFluor488) is designed for detecting mGold fusion proteins specifically. Anti-mGold, AlpSdAbs® VHH(iFluor488) is based on monoclonal, recombinant, single domain antibody to mGold coupled to iFluor488. Based on immunofluorescence and/or ELISA, Anti-mGold, AlpSdAbs® VHH(iFluor488) detects the mGold selectively, no reactivity with other proteins.

Immunogen: mGold
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c
Conjugate: iFluor488(Ex: 495nm, Em: 519nm)
Specificity: mGold, mGold2s, mGold2t
Cross-Reactivity: Recognizes GFP and YFP variants.
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PB(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C, protect from light

Background:
mGold is a yellow fluorescent protein (YFPs), and its variants mGold2s and mGold2t are the brightest and most photostable monomeric yellow fluorescent proteins, breaking the traditional trade-off between photostability and brightness and achieving simultaneous enhancement of both properties. Compared to widely used YFPs such as mVenus and mCitrine, their photostability has increased by 25 times, with minimal changes to other biophysical properties.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Performance

ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Super-resolution microscopy

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

FAQ

Citations