Details and Advantages
Applications:
WB,ELISA,ICC/IF,Flow Cyt
Reactivity:
tdTomato
Conjugate:
iFluor488
Advantages:
High lot-to-lot consistency
Increased sensitivity and higher affinity
Animal-free production
Summary
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Description:
Anti-tdTomato, AlpHcAbs® Rabbit antibody(iFluor488) is designed for detecting tdTomato fusion proteins specifically. Anti-tdTomato, AlpHcAbs® Rabbit antibody(iFluor488) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to tdTomato coupled to iFluor488. Based on immunoelectrophoresis and/or ELISA, Anti-tdTomato, AlpHcAbs® Rabbit antibody(iFluor488) detects the tdTomato selectively, no reactivity with other proteins.
Immunogen: tdTomato
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc(mutation)
Conjugate: iFluor488(Ex: 495nm, Em: 519nm)
Specificity: tdTomato
Cross-Reactivity: Highly selective for tdTomato
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
The tandem dimer Tomato (tdTomato) is a genetically improved version of Discosoma sp. fluorescent protein (dsRed). tdTomato is an extremely bright red fluorescent protein, being one of the brightest red-shifted fluorescent proteins available and six times brighter than eGFP. One of the main advantages of fluorescent proteins is that they do not require substrates or reagents for detection, however most of them do need oxygen for the formation of their chromophores. Besides, fluorescent proteins can be measured without lysing cells, whereas this is difficult for luciferases. One of the biggest disadvantages of fluorescent reporters is that they need to overcome cellular autofluorescence. However, in general cellular autofluorescence is lower for the emission wavelength of the red channel, favoring a red fluorescent protein such as tdTomato.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Anti-tdTomato, AlpHcAbs® Rabbit antibody(iFluor488) is designed for detecting tdTomato fusion proteins specifically. Anti-tdTomato, AlpHcAbs® Rabbit antibody(iFluor488) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain antibody to tdTomato coupled to iFluor488. Based on immunoelectrophoresis and/or ELISA, Anti-tdTomato, AlpHcAbs® Rabbit antibody(iFluor488) detects the tdTomato selectively, no reactivity with other proteins.
Immunogen: tdTomato
Host: Alpaca pacous
Isotype: VHH domain of alpaca IgG2b/2c fused to Rabbit IgG Fc(mutation)
Conjugate: iFluor488(Ex: 495nm, Em: 519nm)
Specificity: tdTomato
Cross-Reactivity: Highly selective for tdTomato
Purity: Recombinant Expression and Affinity purified
Concentration: 1mg/ml
Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Background:
The tandem dimer Tomato (tdTomato) is a genetically improved version of Discosoma sp. fluorescent protein (dsRed). tdTomato is an extremely bright red fluorescent protein, being one of the brightest red-shifted fluorescent proteins available and six times brighter than eGFP. One of the main advantages of fluorescent proteins is that they do not require substrates or reagents for detection, however most of them do need oxygen for the formation of their chromophores. Besides, fluorescent proteins can be measured without lysing cells, whereas this is difficult for luciferases. One of the biggest disadvantages of fluorescent reporters is that they need to overcome cellular autofluorescence. However, in general cellular autofluorescence is lower for the emission wavelength of the red channel, favoring a red fluorescent protein such as tdTomato.
Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Performance
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WB: 1:5000 -1:20000
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ELISA: 1:5000 -1:20000
Flow Cyt: 1:200-1:2000
ICC/IF: 1:200-1:2000
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
