AlpVHHs Nanoselector makes IP/CoIP experiments easier

To solve the problems of taking long time and interface of light and heavy chain, when using the conventional antibodies in IP / CoIP, AlpVHHs has developed Nanoselector series nanobody products for various tag and species.

Nanoselector^® is composed of high-affinity, high-specificity nanoantibodies (VHH) covalently coupled to agarose beads or magnetic beads,which can specifically recognize target protein from various cell extraction such as mammals, plants, bacteria, yeast, insects, etc.

The advantages of Nanoselector over traditional IP experimental methods:

01 Faster (2H)

The Nanoselector series has extremely high affinity, ranging from nM ~ pM, which enables it to bind to the target protein quickly and stably (generally only 5-30 minutes). Nanoselector shortens the traditional 2-day process to less than 2 hours. The time is shortened and the results of multiple tests are stable and reproducible. This can keep the experiment efficient and save experimental time and cost.

02 High Loading (10 times)

Nanobody’s molecular weigh is only one of tenth compared with the convention antibody, which can be coupled to solid phase media at a high density and increasing the loading capacity greatly, 10uL beads can bind 10~20ug of protein.

03 More stability

Nanoselector has extremely high stability because of the simple structure , which can remains stable at 70℃. It can work in physiologically concentrated salt ion solutions, moreover, GFP Nanoselector can tolerate harsh environments such as high salt, acid and alkali. The high stability exhibited by Nanoselctor provides researchers with more possibilities to choose different experimental conditions.

04 Easier-NO Light and heavy chain interference

Nanoselector is developed for the method of site-specifically coupling nanoantibodies to a solid phase carrier which will not be destroyed or detached under various conditions in IP/CoIP experiments, therefore, the he eluted product is not affected by light and heavy chains. Whether it is western blot or MS, there is no interference from traditional light and heavy chains, so it is easy to identify proteins that specifically bind to GFP fusion proteins.

Applications:

In addition, AlpVHHs also provides the following Nanoselector products, you can also check detail in our web https://www.alpvhhs.com/Nanoselector.html