Single-domain antibody (sdAbs) is a type of antibody that lacks the light chain and only has the variable region of the heavy chain. Due to their small molecular weight, they are also called nanobody Despite their simple structure, sdAb can still achieve comparable or even higher affinity for specific antigens than traditional antibody. Compared to traditional antibody, sdAb offer advantages such as smaller molecular weight, greater stability, and ease of recombinant expression. Nanobody is widely used in diagnosis, therapy, affinity purification, and structural elucidation. AlpVHHs specializes in the research and development of nanobody products and has constructed Large Naïve VHH Library based on phage display and yeast display.
Product Description:
To ensure library diversity, AlpVHHs used unimmunized PBMCs from 65 Alpaca, 20 llama, and 20 Bactrian camels as the starting material. Through RNA extraction and cDNA reverse transcription, VHH gene fragments were amplified and constructed into the phagemid vector pComb3XSS. Based on zero-background technology, the library insertion rate reaches 100% and library capacity reaches 3*1011.
Advantages of Naïve VHH Library:
1. Short turnaround time: Nanobody can be rapidly screened against target molecules without the lengthy immunization and library construction process.
2. Suitable for screening antigens that are toxic to alpacas and those with low immunogenicity.
3. Broad epitope coverage: Compared to immunization processes, which can produce epitope bias, natural libraries can cover a wider range of epitopes.
4. Large library capacity: The total library capacity of Naïve VHH Library is as high as 3*1011cfu, with a diversity exceeding 10^9, and the library insertion rate is 100%.
The kit contains:
Products:
AlpVHHs offers three nanobody libraries derived from different species. For more details, please visit our website www.alpvhhs.com.
Code | Description | Applications | Size |
AlpSDAb-P | phage display library for screening | 1mL | |
CamSDAb-P | phage display library for screening | 1mL | |
LlaSDAb-P | phage display library for screening | 1mL | |
CamSDAb-Y | yeast display library for screening | 1mL |