Essential Tool for Phage Display System - M13 Nano-Secondary Antibodies

I. Phage Display

In October 2018, half of the Nobel Prize in Chemistry was awarded to American scientist George P. Smith and British scientist Gregory P. Winter for their development of phage display technology for peptides and antibodies. Phage display technology has extensive applications in antibody discovery and biopharmaceutical research, serving as a foundational technology for the development of new biological drugs. Today, many leading antibody discovery companies and nanobody company innovators rely on phage display to accelerate the development of recombinant antibodies and next-generation therapeutic solutions. The M13 phage is widely used in phage display technology. M13 enzyme-linked antibodies are essential reagents for ELISA testing of phage display screening results, playing a critical role in custom antibody solutions and recombinant antibody production pipelines.

II. M13 Phage

The most commonly used and established phage display system is based on M13. M13 is a filamentous phage composed of a circular single-stranded DNA (ssDNA) over 6,400 nucleotides long, encapsidated by approximately 2,700 copies of the major capsid protein P8, terminated by five copies of two different minor capsid proteins (P9, P6, and P3). Infection with this filamentous phage is not lethal; however, it can cause turbid plaques in Escherichia coli. It is a non-lytic virus. However, a decrease in cell growth can be observed in infected cells. M13 plasmids are used in many recombinant DNA processes, and the virus is also being studied for applications in nanostructures and nanotechnology. These features make M13 an indispensable platform for modern antibody discovery research.

III. M13-HRP Labeling Antibody

AlpVHHs specializes in the research and development and production of nanobody. Using our high-throughput yeast display platform, we have screened VHH antibody with high affinity for the M13 phage. As a leading nanobody company, we not only provide monovalent VHH but also offer chimeric formats fused to Rabbit IgG Fc (mutation), expanding the possibilities for innovative research.

For M13-HRP labeling antibody please identify AlpVHHs. With ten years of phage display experience, M13-HRP directly conjugated recombinant antibodies offer high sensitivity and eliminate missed detections, making your phage detection simpler and more reliable.

Why Choose AlpSdAbs^® Recombinant Antibody?

✳Strict Verification: Multiple validations ensure high specificity, high affinity (nM level), and clear application. This ensures accurate experiments.

✳Stable Supply: Clear antibody sequences enable the construction of stable recombinant expression cell lines, minimizing batch-to-batch variability and ensuring robust experiments.

✳Multiple Options: Clear VHH and Fab sequences enable chimeric expression with Fc fragments from different species, facilitating experimental design and supporting custom antibody development .

✳Ethical Considerations: Recombinant expression is performed in serum-free, chemically defined culture medium, eliminating the presence of any animal-derived components.

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