Why Choose Nanoselector for HA IP?

Foreword

Dear research partners, we understand that the success and efficiency of IP experiments directly impact your research progress. Traditional IP processes are like a marathon, not only time-consuming and labor-intensive but also introducing numerous uncertainties. AlpVHHs’ blockbuster product HA tag Nanoselector Magnetic beads (Code: 003-101-003), aims to completely liberate you from tedious and lengthy experimental procedures, redefining the speed and reliability of IP experiments with "ultimate simplification" and "ultimate efficiency"!

Core Disruption: What is the "5μL or 10min" Revolution?

● Advantage 1: Ultra-high load capacity, high efficiency with minimal volume. 

Only 5μL of magnetic beads are needed to efficiently complete a standard IP experiment. Less usage, higher load capacity, minimal volume for ultimate cost savings.

Fig 1. Test with different amounts of HA magnetic beads.

● Advantage 2: Rapid Incubation, Quick and Stable Accumulation.

Only 10 minutes of incubation is needed to achieve highly efficient enrichment of HA-tagged proteins. High speed and efficiency eliminate the long wait times of traditional methods (hours or even overnight), significantly shortening the experimental cycle. Combined with HRP direct-labeling antibody, result can be obtained in just one day.

Fig 2. Test of HA magnetic beads at different incubation times

● Advantage 3: Gentle elution, protecting protein activity. 

Only 100μg/mL of HA Peptide (Code: APK053) is needed for gentle and effective elution. No harsh elution conditions are required, protecting protein activity and structural integrity, ensuring the reliability of downstream WB, IP/Co-IP, or mass spectrometry analysis data.

Fig 3. Competitive elution concentration test of HA Peptide

Proof of Strength: Data Speaks for Itself

We firmly believe that accurate and rigorous data is the most powerful proof of product performance.

Fig 4. Result of P53 immunoprecipitation

Test condition: AlpVHHs' HA magnetic beads (25μL), brand L HA magnetic beads (40μL), and brand S HA magnetic beads (25μL) were used, and P53 immunoprecipitation assays were performed simultaneously.

Result: Western blotting result showed that AlpVHHs’ HA magnetic beads had high enrichment efficiency and strong specificity, presenting only a single clear target protein band, with performance comparable to or even better than similar products.

Fig 5. Effect of Ssnlp1/flg22 on CNGC3 phosphorylation (Yi H, et al., 2025)

Test condition: Immunoprecipitation was performed using HA tag Nanoselector Magnetic beads (Code: 003-101-003).

Result: The team led by Cai Xinzhong at the Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, obtained a single, clear target protein band in plant samples using HA tag Nanoselector Magnetic beads (Code: 003-101-003). The band exhibited high enrichment efficiency and excellent specificity. Their findings were published in the journal *Molecular Plant* (IF=24.1/Q1) under the title "BIK1-mediated phosphorylation and SCFSKIP31-mediated ubiquitination coordinately control CNGC3 homeostasis and activity to fine-tune plant immunity".

Why are our magnetic beads so outstanding?

Unveiling the core nanobody technology behind their superior performance

Nanoselector^® magnetic beads are composed of nano-tagged antibodies (VHH) covalently bound to magnetic agarose beads. They are used for immunoprecipitation of tagged fusion proteins from cell extracts of various organisms, including mammals, plants, bacteria, yeast, and insects, offering advantages such as high loading capacity and no light or heavy chain interference. Creating a stable and reusable recombinant antibody platform with unmatched performance.

The path of scientific exploration is fraught with challenges, and a reliable tool is key to success. AlpVHHs, as an innovative antibody discovery company, is committed to empowering researchers worldwide with high-performance nanobody, VHH antibody, and recombinant antibody solutions. The HA tag Nanoselector Magnetic beads (Code: 003-101-003) is more than just a product; it represent a pure, efficient, and worry-free experimental solution for you.

Act now! Say goodbye to long waits and uncertain results. Unlock your experimental “speed mode” with Nanoselector and let your next scientific breakthrough start with a perfect IP/Co-IP experiment!

Visit our website www.alpvhhs.com for more production antibodies and antibody discovery.