Why Choose Nanoselector for Flag IP?

Flag Nanoselector has sold over 5000 units.

Dear research partners, we understand that the success and efficiency of IP experiments directly impact the progress of your research. Traditional IP processes are like a marathon, not only time-consuming and labor-intensive but also introducing numerous uncertainties.

AlpVHHs’ blockbuster product, DYKDDDDK tag Nanoselector Magnetic beads (Code: 016-101-003), aims to completely liberate you from tedious and lengthy experimental procedures, redefining the speed and reliability of IP experiments with "ultimate simplification" and "ultimate efficiency"!

Core Disruption: What is the "5μL or 10min" Revolution?

Advantage 1: High Load Capacity 

Only 5μL of magnetic beads are needed to efficiently complete a standard IP experiment. Fewer magnetic beads mean higher load capacity, achieving ultimate micro-capacity and cost savings.

Fig 1. Test with different amounts of magnetic beads

Advantage 2: Fast and Stable Capture 

Only 10 minutes of incubation is needed to achieve highly efficient enrichment of Flag-tagged proteins. This high-speed and efficient method far surpasses the lengthy waiting times of traditional methods (several hours or even overnight), significantly shortening the experimental cycle. Combined with HRP direct-labeling antibodies, results can be obtained in just one day.

Fig 2. Test of HA magnetic beads at different incubation times

Advantage 3: Convenient Elution

Effective elution can be achieved using only 100 μg/mL of 3×Flag Peptide (Code: APK051). No harsh elution conditions are required, protecting protein activity and structural integrity and ensuring the reliability of downstream applications such as Western blotting, IP/Co-IP, or mass spectrometry.

Fig 3. 3×Flag Peptide Competitive Elution Concentration Test

Evidence of Strength: Let the Data Speak

We firmly believe that accurate and rigorous data is the most powerful proof of product performance.

Fig 4. Tag-fused protein enrichment (client feedback image)

Test conditions: Flag-tagged proteins were precipitated from complex cell lysates using 25 μL magnetic beads.

Results: Western blotting results showed that our magnetic beads yielded single, clear target protein bands in the samples with high enrichment efficiency and excellent specificity.

Fig 5. Result of P53 and MDM2 immunoprecipitation

Test conditions: Immunoprecipitation assays for P53 and MDM2 were performed simultaneously using critical point Flag beads (25 μL) and brand L Flag beads (40 μL).

Results:

1. Detection of bait protein P53: The critical point enrichment was significantly higher than that of brand L, being 2.8 times higher.

2. Detection of prey protein MDM2: P53 and MDM2 interacted, and the critical point interaction binding was significantly higher than that of brand L, being 9.8 times higher.

Why are our magnetic beads so outstanding?

Unveiling the core nanobody technology behind their superior performance

Nanoselector^® magnetic beads are composed of nano-tagged antibodies (VHH) covalently bound to magnetic agarose beads. They are used for immunoprecipitation of tagged fusion proteins from cell extracts of various organisms, including mammals, plants, bacteria, yeast, and insects, offering advantages such as high loading capacity and no light or heavy chain interference. Creating a stable and reusable recombinant antibody platform with unmatched performance.

The path of scientific exploration is fraught with challenges, and a reliable tool is key to success. AlpVHHs, as an innovative antibody discovery company, is committed to empowering researchers worldwide with high-performance nanobody, VHH antibody, and recombinant antibody solutions. The DYKDDDDK tag Nanoselector Magnetic beads (Code: 016-101-003) is more than just a product; it represent a pure, efficient, and worry-free experimental solution for you.

Act now! Say goodbye to long waits and uncertain results. Unlock your experimental “speed mode” with Nanoselector and let your next scientific breakthrough start with a perfect IP/Co-IP experiment!

Visit our website www.alpvhhs.com for more production antibodies and antibody discovery.