Mouse Antibody lsotyping Kit
Mouse Monoclonal Antibodies
In 1975, British scientist Milstein and French scientist Kohier fused antibody-producing B lymphocytes with mouse myeloma cells, thereby establishing the first B-cell hybridoma cell line. Hybridoma cells possess the characteristics of their parental cells, capable of producing antibodies while exhibiting the unlimited proliferation properties of tumor cells, enabling continuous secretion of monoclonal antibodies. In 1986, Orthoclone OKT3 became the first monoclonal antibody drug to be approved by the U.S. FDA for the prevention of host rejection following kidney transplantation. Orthoclone OKT3, derived from mice, represented the first generation of murine monoclonal antibodies. Mouse monoclonal antibodies are produced through a complex process involving the fusion of B cells from immunized mice with myeloma cells. This is followed by a stringent selection process that identifies mouse hybridoma cells capable of both unlimited proliferation and antibody secretion. These cells are then subjected to a subsequent screening, preparation, and purification process. Beyond the possession of general properties of antibodies, mouse monoclonal antibodies exhibit structural and compositional homogeneity, target a single antigenic determinant, and offer advantages such as high specificity and excellent stability. These instruments find wide application in fundamental research, medical diagnostics, and numerous other fields.
Identification of Mouse Monoclonal Antibody Subtypes
Mice possess five antibody isotypes (IgA, IgD, IgE, IgG, and IgM), each with distinct heavy chains. Mouse IgG constitutes 75% of serum immunoglobulins, serving as the primary antibody produced by mice. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in primary immune responses. Mouse IgG comprises five subclasses: IgG1, IgG2a, IgG2b, IgG2c (inbred mice carrying the Ig1-b allele express IgG2c instead of IgG2a), and IgG3. These subclasses exhibit high homology, with differences primarily in the heavy chain hinge region. The entire IgG molecule possesses Fc and Fab regions, with the Fab region containing epitope recognition sites. The IgG molecule consists of a pair of heavy chains and light chains (k or λ).
Currently, most monoclonal antibodies originate from mice. Identifying the subclass of mouse monoclonal antibodies is crucial for clarifying their physicochemical properties, facilitating downstream purification, and ensuring proper antibody storage and usage.
Isotyping Kit for Mouse Monoclonal Antibody
Chengdu AlpVHHs Co., Ltd (AlpVHHs) specializes in the research, development, and production of nanobodies. Using a high-throughput phage display platform, we have screened for nanobodies with high affinity and specificity for mouse subtypes. By fusing monovalent nanobodies with Goat Fc(mutation), the affinity is further enhanced, and the Fc(mutation) removes potential interference with cell receptor binding. The Isotyping Kit for Mouse Monoclonal Antibody (APKO01) is a powerful research tool for the qualitative determination of mouse immunoglobulin subclasses. This kit accurately identifies mouse immunoglobulin subclasses, including IgG1, IgG2a, IgG2b, IgG3, IgM, and kappa, from hybridoma cell culture supernatants or purified antibodies via enzyme-linked immunosorbent assay (ELISA). The Isotyping Kit for Mouse Monoclonal Antibody (APKO01) consists of goat Fc-fused nanobodies targeting mouse antibody subclasses, offering higher specificity and sensitivity than most existing products.
All Products
| Code | Description | Applications | Size |
| APK001 | Isotyping Kit for Mouse Monoclonal Antibody | ELISA |
